Blocking Solutions

Save ¥1,300

Sale price¥11,700 Regular price¥13,000

Shipping calculated at checkout

In stock
  • 1.Transportation and storage conditions are shown as T/S code (two alphabets and a number) of each product. The meaning of T/S code is as follows.  Initial alphabet: Storage condition after product arrival (A: 10~35 ˚C, B: 2 to 8˚C、C: below -15 ˚C) Second alphabet: Transportation condition (A: 10~35 ˚C, B: 2 to 8˚C、C: below -15 ˚C)
    Last number: Number of products packable in a minimal size parcel.
  • 2. For customers of outside Japan: This site is designed to charge transportation cost to each single order. So, if you would like order multiple number of products (including multiple order of the same products) and wish to minimize transportation cost, we recommend to order them from our other site, by clicking “contact” form.
  • 3. If you wish to purchase products that may exceeds more than 20kg dimensional weight, please contact us from our original site (

  • 1. 注文頂いた製品の輸送は各製品に記載した輸送/保存コード(2つのアルファベットと数値)に基づいて行います。各アルファベットと数値の意味は以下の通りです。 最初のアルファベット:到着後の保存温度(A:常温保存、B:冷蔵保存、C:冷凍保存) 2番目のアルファベット:輸送時の温度(A:常温輸送、B:冷蔵輸送、C:冷凍輸送) 3番目の数値:一つのパッケージで輸送可能な製品数(輸送費はこの範囲の数であれば同じです)
  • 2. 国外からの注文の場合:本サイトからの注文ではシステム上1つの製品毎に輸送料が必要となります。複数個(同一製品の複数個の注文含む)を購入する場合で、送料を抑えたい時は、の“contact” form から注文されることをお勧めします。
  • 3. 20kg以上の製品を海外に送る場合の送料に場合には、当社オリジナルサイトの問合せフォーム(からお問合せ下さい。


Product outline

As blocking solution, we developed 4 types of Blocking Solutions for Western blot and ELISA. Blocking is used to minimize non-specific binding of antigens or antibodies to the surface of membrane or ELISA plate by covering blocking agents. However, the property of antigens or antibodies is different each other, and the non-specific binding also differ depending on detection methods, the selection of blocking agents is very important to get best results.

Beacle developed blocking solutions specific for Western blot or for ELISA, which contain 4 types of different blocking agents. The blocking solutions for ELISA are designed to long-term preservation of pre-coated plate.

Products with last code of “-w” are designed for Western blot, and “-e” for ELISA. 

flyer for Blocking solutions

Selection of Blocking agents

Blocking Solutions have following main ingredients and characteristics ;
c-Block:100% chemical components. Good for WB and antibody-detecting ELISA.
h-Block:Casein-based. Good for many applications.
k-Block:degnatured casein-based. Shows stable results in many applications.
b-Block:BSA-based. Good for many applications.
Trial set:a trial kit composed of all the above 4 solutions. Good for screening of best blocking solution

Selection of Blocking agents
A general rule is described. We strongly recommend to select the best one by screening using Trila Set.
For Western Bot Many reserachers seems to use Skim milk. Skim milk is not bad choice, but it very often gives you weak signal due to its strong blocking action. Below is the recommendated use of our bloking solutions.
①To get higher signal(accept some background increase):k-Block, c-Block
②To reduce background(accept some decrease of signal):b-Block, h-Block
③To use as a standard blocking:h-Block, b-Block
④To detect phospho-protein:c-Block, b-Block
For ELISA Below shows choice of blocking solutions depending on the ELISA system you use. All the solutions for ELISA are desinged for long term preservation, and dryed precoated plate can be stored for a few months after blocking at 4℃.
Antigen-detecting ELISA:
 Direct method (detect antigen adsorbed on plate by detecting antibody):c-Block, b-Block, k-Block
 Antibody-sandwich method (detect antigen by captured antibody by detect antibody):k-Block, b-Block, h-Block
Antibody-detecting ELISA:
 Direct method (detect antibody captured by pre-coated antigen):c-Block, b-Block, k-Block
 Antigen-sandwich method (detect antibody by captured antigen by detect antigen):k-Block, b-Block, h-Block 

Product feature

1. Ready-to-Use
2. Optimized blocking solution for WB and ELISA
3. For WB, stronger signal can be obtained by using Skim milk*
4. For ELISA, pre-coated plate can be stored for months

*: based on experiments in our laboratory

Estimate shipping

You may also like

Recently viewed